Abstract: OBJECTIVE To explore and establish a new and practical hemorrhoids model which is relatively closer to the actual pathological symptoms of human hemorrhoids. METHODS According to the pathological characteristics of human hemorrhoids, such as local varicose veins of the anal canal, blood stasis, microcirculation disorder and local inflammation, combined with the principles of pharmacology, chose several drugs and reagents including microcirculation blockers, intestinal smooth muscle movement inhibitors, pro-inflammation and swelling reagents, at appropriate doses administrated together via local subcutaneous injection in anal canal, to induce hemorrhoids symptoms in SD rats. RESULTS After the 2-week modeling experiment period and the subsequent observations, this model-building method produced a series of symptoms similar to the clinical symptoms of human hemorrhoids, such as inflamed crissum and constipation, and significantly increased the crissum diameter of the modeling animals. In the histological and pathological observations of the anal canal of rats, the pathological changes including vessels hyperplasia, thrombus, haemorrhoids, inflammation and granulomatous were observed in the animal model, which could well simulate and reflect the pathological symptoms of human hemorrhoids. CONCLUSION This method can establish a stable and reliable animal model of hemorrhoids with manifestations of the clinical disease symptoms.