Abstract: OBJECTIVE To further improve perfectly the current quality standard from Uighur medicine Viola Tianshanicae. METHODS The pharmacognostical methods were carried out through morphological, microscopic characteristics and TLC. HPLC was used to determine the content of aesculetin from Uighur medicine Viola Tianshanicae. The Agilent TC-C₁₈(4.6 mm×150 mm, 5 μm) column was used with acetonitrile-1% acetic acid solution (15:85) as the mobile phase at a flow rate of 0.8 mL·min^-1. The detection wavelength was set at 349 nm of aesculetin. The column temperature was 35℃. The alcohol-soluble extracts content, water, ash and acid insoluble ash from Uighur medicine Viola Tianshanicae were determined respectively by the methods issued in the Pharmacopoeia (2015 edition) of the People's Republic of China. RESULTS The results showed that the proper characters and the microscopic characteristics from Uighur medicine Viola Tianshanicae were described exclusivly. Qualitative identification for all the ingredients from Uighur medicine Viola Tianshanicae of 10 batches commercial was carried out by TLC. Aesculetin was linear over the range of 0.1-3.0 μg·mL^-1(r=0.999 3), the recovery rate was 98.56%, RSD was 1.90%. The inspecting limit of alcohol-soluble extracts content, water, ash and acid insoluble ash from Uighur medicine Viola Tianshanicae of 10 batches commercia had been identified through experimental results. CONCLUSION The methods is simple, accurate and steady for further improvment perfectly the current quality standard of Uighur Medicine Viola Tianshanicae, can be used for the quality control of Uighur Medicine Viola Tianshanicae.