Abstract: OBJECTIVE To prepare a novel anti-liver injury compound XXH-32 liposome and study its pharmaceutical properties. METHODS The liposome was prepared by thin-film dispersion method. The optimized preparation process was investigated by a series of experiments under orthogonal design. The surface morphology of the liposome was observed by scanning electron microscopy. The particle size and drug loading of the liposome was measured, and XXH-32 release behavior from the liposome was studied. RESULTS Under the optimal preparation process, the liposome was presented round in shape with average diameter of (175.2±2.55)nm, polymer dispersity index was 0.262±0.01. The content of XXH-32 in the liposome was (2.60±0.12)%, and the entrapment efficiency was (95.05±1.06)%. The release of XXH-32 from the liposome presented a first-order kinetic behavior. The equation as follow ln(100-Q)=-0.06t+4.79, R²=0.979 4. CONCLUSION XXH-32 liposome was prepared successfully with high drug entrapment efficiency. XXH-32 in the liposome showed a significantly slower release behavior.