Abstract: OBJECTIVE To establish high performance liquid chromatography(HPLC) fingerprints of Fufang Siyadan Oil to provide a reference for evaluation of the overall quality of this preparation. METHODS The similarity of the HPLC fingerprint of Fufang Siyadan Oil analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2012A", and hierarchical clustering analysis(HCA) was performed by SPSS 19.0. RESULTS There were 8 common peaks, and the similarity degrees of 10 batches of samples were >0.95, and showed that all the samples were of good consistency. The samples were divided into 4 clusters by HCA. CONCLUSION Establishment of fingerprint can provide more comprehensive reference for the quality control of Fufang Siyadan Oil. The established HPLC fingerprint method is stable and feasible.