Abstract: OBJECTIVE To establish a method to determine the content of 5 active ingredinents of costunolide, dehydrocostuslactone, naringin, neohesperidin and hesperidin in Xiangshazhizhu pills, and investigate the changes of the content of effective ingredinents before and after ⁶⁰Co-γ ray irradiation. METHODS The Agilent-C₁₈ column(4.6 mm×250 mm, 5 μm) was adopted and the detection wavelength was 225 nm with the flow rate of 1.0 mL·min^-1. The mobile phase was consisted of acetonitrile-0.5% phosphoric acid with a gradient elution, and column temperature was 35℃. The irradiation dose of 2, 5 and 8 kGy were selected to irradiate Xiangshazhizhu pills, and the content of 5 active ingredinents in Xiangshazhizhu pills before and after irradiation was compared, and the significant situation was observed by group t-test. RESULTS The linear range of costunolide, dehydrocostuslactone, naringin, hesperidin and neohesperidin were 0.060 6-3.032 5, 0.025 8-1.290 0, 0.037 3-1.865 0, 0.044 2-2.210 0 and 0.033 6-1.680 0 μg, and the average recovery were 100.6%, 101.0%, 99.7%, 100.2% and 99.5%, RSD were 0.5%, 1.3%, 0.8%, 1.0% and 0.7%. After irradiation of 2, 5 and 8 kGy, the effective ingredinents of costunolide, dehydrocostuslactone, naringin, hesperidin and neohesperidin were changed. Over 5 kGy, costunolide and dehydrocostuslactone according to change of content before and after irradiation was statistically significant(P<0.05) by group t-test. CONCLUSION The established method has a high recovery rate, good repeatability, is simple and practical and can be used for quality control for Xiangshazhizhu pills. When the radiation dose is ≤ 5 kGy, the changes of each component are not significant, which can provide a reference for the sterilization of Xiangshazhizhu pills.