Abstract: OBJECTIVE To develop a method of quantitative analysis of multi-components by single marker(QAMS) for six index components in Zhengtian pills. METHODS The method with a Agilent XDB C₁₈ column(250 mm×4.6 mm, 5 μm) was applied, the mobile phase was acetonitrile-0.1% phosphoric acid of gradient elution, the flow rate was 1.0 mL·min^-1,the detection wavelength was 310 nm,the column temperature was 30℃ and the injection volume was 10 μL. Used paeoniflorin as a reference, the relative correction factors among the six kinds index components were detected by QAMS. These six index contents were determined by external standard method and QAMS. The results obtained from two different methods were compared. Verified the practicability and stability of QAMS. RESULTS The established QAMS was used to determine the six index components in Zhengtian pills, and totally six batches of Zhengtian pills were determined. There was no significant difference in the calculated values and the measured values. CONCLUSION The QAMS method is simple, effective and accurate in determining the contents of six index components in Zhengtian pills, which can be used for the quality control of Zhengtian pills and provide reference for the improvement of quality standards.