Abstract: OBJECTIVE To study the inhibitory effects and mechanism of Jiawei Xiaoxianxiong formula granule on hypertrophic scar tissue of rabbits and to provide experimental evidence for its clinical application. METHODS The hypertrophic scar model in rabbit ears was established. The rabbits were randomly divided into 4 groups including control, model, low-dose and high-dose groups four rabbits in each group. On the 21st day after the operation, the model group was given normal saline, and the corresponding drugs in the low-and high-dose administration groups were once a day. After 60 days, the morphological and pathological changes of the scar tissue were examined. And the levels of TGF-β, PDGF-BB, CTGF, Collagen Ⅰ and MMP-2 were detected. RESULTS Compared with the model group, hypertrophic index(HI) in low-dose group was significantly decreased, while the difference of HI in high-dose group was not significantly. HE and Masson staining showed the obvious scar hyperplasia, disordered collagenous arrangement and rotation-like appearance in the model group, while orderly collagenous arrangement and a small amount of microvessels in drug intervention groups. Compared with the control group, the contents of TGF-β, PDGF-BB and CTGF in models were increased significantly, and the expression of Collagen Ⅰ protein and mRNA were also significantly increased accompanied by significantly decreased expression of MMP-2. Compared with the model group, the expression of TGF-β and Collagen Ⅰ was significantly decreased while the expression of MMP-2 was significantly increased in drug intervention groups. CONCLUSION Jiawei Xiaoxianxiong formula granule could inhibit the formation of hypertrophic scar in rabbit ears, and its mechanism may be related to the inhibition of the expression of TGF-β, PDGF-BB and CTGF.