Abstract: OBJECTIVE To study the effect of Angelica sinensis stems and leaves and Angelica sinensis roots on hemolytic anemia model mice. METHODS Ninety mice were randomly divided into 9 groups:control group, model group, positive group, and Angelica sinensis stems and leaves(high, middle and low dose) and Angelica sinensis roots(high, middle and low dose) groups, 10 mice in each group, half male and half female. The animal model of hemolytic anemia was made by using acetyl phenyl hydrazine(APH)(1, 4, 7 d hypodermic inject saline containing 0.2, 0.1, 0.1 g·kg^-1 APH, respectively). On the first day of modeling, Angelica sinensis roots decoction, Angelica sinensis stems and leaves decoction were given to mice by gavage(0.02 mL·g^-1). Samples were taken 13 d later to test their blood routine(Hb, RBC, PLT), organ index(spleen index, thymus index), biochemical indicators(G₆PD, GR, IL-2 and EPO), and to observe pathological sections of liver and spleen with HE staining. RESULTS Compared with the model group, Angelica sinensis stems and leaves, roots decoction could significantly increase Hb, RBC, PLT, decrease spleen index and improve the thymus index. Besides, increased the levels of G₆PD, GR, IL-2 and EPO in the blood of mice(P<0.05 or <0.01). In addition, the results of pathological sections showed that Angelica sinensis stems and leaves decoction, Angelica sinensis roots decoction both reduced the point necrosis and congestion of liver and spleen cells in mouse induced by APH. CONCLUSION The Angelica sinensis stems and leaves, and Angelica sinensis roots have improving effect on hemolytic anemia mice.