纤维素键合手性固定相拆分盐酸奈福泮对映体

Separation of Nefopam Hydrochloride with a Bonded Cellulose Chiral Stationary Phase

摘要: 目的建立一种用键合纤维素手性固定相的HPLC拆分盐酸奈福泮对映异构体。方法盐酸奈福泮的分离使用Chiralpak IC色谱柱（250 mm×4.6 mm，5 μm），流动相为正己烷-异丙醇（90：10，0.1% DEA），流速为0.8 mL·min^-1，检测波长267 nm，柱温25℃。结果盐酸奈福泮对映体在键合纤维素手性固定相上能够完全分离，分离度为6.53。结论该方法可快速实现盐酸奈福泮对映体的分离。

Abstract: OBJECTIVE To develop an HPLC method with a bonded cellulose chiral stationary phase for the chiral separating enantiomers of nefopam hydrochloride. METHODS Nefopam hydrochloride was separated by a Chiralpak IC (250 mm×4.6 mm, 5 μm) at 25℃ and detected at 267 nm, the mobile phase was n-hexane-isopropanol(90:10, 0.1% DEA) at a flow rate of 0.8 mL·min^-1. RESULTS Nefopam hydrochloride enantiomers were separated on a cellulose bonded chiral stationary phase and the resolution was 6.53. CONCLUSION The method can be used for quickly separating the enantiomers of nefopam hydrochloride.