Abstract: OBJECTIVE To explore the methods and precautions for the concentrated deployment of cytotoxic drugs and reduce the potential physical damage to medical personnel. METHODS Based on the drug specification and relevant pharmacy data, the most suitable method for the preparation of cytotoxic drugs was analyzed, and the solvent, the amount of drug, the rate of drug infusion, the time limit for drug administration, and the precautions for infusion were determined. RESULTS The methods for the preparation of various cytotoxic drugs, precautions for use, and potential risks of drug use were clarified, and the procedures were strictly followed to ensure the safety of the drug. CONCLUSION The normative method and operation of cytotoxic drug deployment are important measures for the safe use of drugs and the reduction of accidental injuries of medical personnel.