Abstract: OBJECTIVE To compare the content differences of chlorogenic acid, macranthoidin B and dipsacoside B in floral buds at different developmental stages of Lonicera macranthoides Hand. -Mazz. METHODS The chromatographic column was CAPCELL PAK C₁₈ MG (250 mm×4.60 mm, 5 μm); the moblie phase was acetonitrile (A)-0.4% acetic acid (B) with gradient elution; flow rate was 1.0 mL·min^-1; UV detection wavelength was 330 nm; ELSD testing condition was as follows:tube temperature 110℃, gas flow 3.0 L·min^-1 and impactor off; column temperature was 30℃. Using HPLC-UV-ELSD series technology, the contents of three components in floral buds collected at different developmental stages (youlei stage, sanqing stage, dabai stage, yinhua stage, jinhua stage, diaohua stage) in Lonicera macranthoides Hand. -Mazz were measured. The content of chlorogenic acid was calculated by outside the two point method, and the content of macranthoidin B and dipsacoside B was calculated by outside the two point logarithmic equation method. RESULTS The contents of chlorogenic acid and dipsacoside B were 6.934% and 1.800%, respectively, were the highest at sanqing stage in Lonicera macranthoides Hand. -Mazz.. However, the highest content of macranthoidin B was 9.351% at youlei stage, which was 0.227% higher than that at sanqing stage. CONCLUSION By comparing the contents of chlorogenic acid, macranthoidin B and dipsacoside B in floral buds at different developmental stages in Lonicera macranthoides Hand. -Mazz., sanqing period is the best harvest time with high content.