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    UPLC同时测定中成药和保健食品中16种非法添加的抗过敏类药物

    Determination of 16 Anti-allergic Drugs in Chinese Traditional Patent Medicine and Health Food by UPLC

      摘要
    • 摘要: 目的 建立UPLC测定中成药和保健食品中16种非法添加的抗过敏类药物。方法 采用Waters BEH C18色谱柱（100 mm×2.1 mm，1.7 μm），以甲醇-20 mmol·L-1磷酸二氢钠（用磷酸调节pH值至3.0）为流动相，梯度洗脱，检测波长为220 nm。结果 16种抗过敏类药物在2.5~40 μg·mL-1内呈良好线性关系（r2>0.999），平均回收率为90.77%~113.35%，RSD为0.06%~1.21%；定量限为0.18~3.92 μg·mL-1，检出限为0.09~0.78 μg·mL-1。结论 该方法简便、灵敏度高，可有效地判断中成药及保健品中是否非法掺入16种抗过敏类药物。

       

      Abstract: OBJECTIVE To develop a UPLC method to determine 16 anti-allergic drugs illegally added in chinese traditional patent medicine and health food. METHODS The separation was performed on a Waters BEH C18 column (100 mm×2.1 mm, 1.7 μm) with the mobile phase consisted of methanol-20 mmol·L-1 sodium dihydrogen phosphate solution (adjusted to pH 3.0 with phosphoric acid) in a gradient elution mode, the detector wavelength was 220 nm. RESULTS The results of 16 anti-allergic drugs showed good linearity in the range of 2.5-40 μg·mL-1(r2>0.999). The average recoveries were from 90.77% to 113.35%, with the RSD from 0.06% to 1.21%. The limits of quantifications were among 0.18-3.92 μg·mL-1, and the limits of detections were among 0.09-0.78 μg·mL-1. CONCLUSION This method is simple and sensitive, and it can effectively judge whether the chinese patent medicines and health products are illegally mixed with these 16 anti-allergic drugs.

       

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