Abstract: OBJECTIVE To evaluate the effect of the whole recipe of Xiaoer Jianpi cream and its disassembled prescription on the stomach muscle and ileum smooth muscle of guinea-pig, and the effect of ingredient on promoting gastrointestinal motility and its relationship with whole recipe is further studied. METHODS Biological functional system was used to record the effects of the whole recipe and its disassembled prescription on the contraction activity in stomach muscle and ileum smooth muscle of the normal guinea-pig. RESULTS The whole recipe had the effects on promoting contraction of ileum and stomach muscle. In its disassembled prescription, clove, evodia and hawthorn could increase the contractile amplitude in stomach muscle significantly, the effects of cinnamon, white pepper were not obvious. Clove and hawthorn could increase the contractile amplitude in ileum smooth muscle of guinea-pig significantly, clove > hawthorn > the whole recipe, the evodia showed an inhibitory effect, and the effect of cinnamon and white pepper was not significantly. The two part of the recipe by orthogonal method which had promoting effect on the gastric muscle were clove+cinnamon, clove+white pepper, evodia+ cinnamon, evodia+white pepper and hawthorn+cinnamon. The two part of the recipe by orthogonal method which had promoting effect on the ileum smooth muscle were clove + cinnamon, while clove + white pepper, hawthorn + cinnamon; evodia + cinnamon, evodia + white pepper showed significant inhibitory effect. CONCLUSION the Xiaoer Jianpi cream can promote the contractile amplitude in stomach muscle and ileum of guinea-pig significantly, clove and hawthorn are the main ingredient of promoting gastrointestinal motility.