Abstract: OBJECTIVE To introduce the research progress and application developments of capillary microemulsion electrokinetic chromatography(MEEKC) in recent years, and provide an useful reference for the application of this technology in drug analysis. METHODS Retrieving and reading research reports of MEEKC to summarize the applications of microemulsion electrokinetic chromatography in the determination of N-octanol-water partition coefficient (logP), separation of natural products and chiral compounds. RESULTS MEEKC had significant advantages for the separation of poorly soluble substances and complex mixtures. In the meanwhile, it was one of the most convenient and accurate chromatographic method for determining logP values. However, microstructure analysis of microemulsion still need further research. CONCLUSION MEEKC combines the features of both chromatographic and electrophoretic separations. It has a wide range of application scope and can separate water-soluble, fat-soluble, acidic, basic and neutral substances simultaneously. Furthermore, the stability and reproducibility of capillary microemulsion electrochromatographic analysis still need to be improved.