Abstract: OBJECTIVE To establish drug use evaluation(DUE) criteria for vancomycin and teicoplanin, to evaluate clinical use of glycopeptides antibiotics in a hospital of the three levels of first class. METHODS Based on vancomycin and teicoplanin instructions, referring to related specifications and literatures, DUE criteria for vancomycin and teicoplanin were established. The utilization of glycopeptides antibiotics in 274 inpatients of the hospital during Jan 2017-Jun 2017 was evaluated in retrospective study. RESULTS The treatment effective rate of glycopeptides antibiotics was 75.91%, the coincidence rate of medication indication was 92.70%, the rate of accuracy dosage was 98.18%, the rate of accuracy administration was 91.24%, the rate of rational duration of treatment was 79.93%, the rate of rational combined drug was 72.26%. CONCLUSION Established DUE criteria of vancomycin and teicoplanin can standardize the clinical utilization of glycopeptides antibiotics, clinical use of vancomycin and teicoplanin is basically reasonable in this hospital.