Abstract: OBJECTIVE To study the anti-cancer mechanism of three main components in Kanglaite injection by using network pharmacology. METHODS Triolein, coixenolide, coixol were screened from Kanglaite injection as the representative compound. Anticancer targets of Kanglaite injection were predicted and screened relying on traditional Chinese medicine pharmacology system technology platform (TCMSP) database, reverse molecular docking server(PharmMapper), annotation of the human genome database (GeneCards) and Drug treatment target database(TTD). The Cytoscape 3.5.1 software was used to construct active ingredients-targets-diseases network. The STRING platform was used to construct protein-protein interaction network. And Biological information annotation databases(DAVID) was used to analyze metabolic pathways and biological processes of the targets. RESULTS Kanglaite injection acted on 25 cancer targets, regulated FoxO, prostate cancer, cancer, Rap1, TNF, Ras, MAPK metabolic pathways and were mainly involved in regulation of cell proliferation, regulation of protein kinase B signaling, cyclooxygenase pathway, regulation of cell migration, could be used for more than 20 cancers. CONCLUSION It provides a new clues and ideas for further in-depth studying on anti-cancer mechanism of Kanglaite injection.