Abstract: OBJECTIVE To synthesis and characterize the chitosan-porphyrin complexes(CS-BHP), and to optimize the experimental condition. METHODS CS-BHP was synthesized from 5-p-(6-bromohexylaminophenyl)-10,15,20-triphenyl porphyrin(BHP) and chitosan by using K₂CO₃ as a base and tetrabutylammonium bromide as a phase transfer catalyst. The porphyrin grafting rate of CS-BHP was detected with ninhydrin by UV-Vis. In the single factor analysis, the effect of temperature, reaction time, the volume ratio of water to chloroform in the component solvent, equivalent of the catalyst were investigated. The orthogonal test was used to optimize the condition of synthesis. RESULTS The optimized condition of the reaction was as follows:the temperature was 55℃, the reaction time was 5.5 h, the volume ratio of water to chloroform in the component solvent was 1:2, catalyst was 1 equivalent, and K₂CO₃ was used as base, the average porphyrin grafting rate of CS-BHP was 41.30%. CONCLUSION It is feasible to synthesize CS-BHP by the method of covalent coupling.