Abstract: OBJECTIVE Using the method of network pharmacology to analyze the mechanism of active ingredients in Zengye decoction, and to explore the correlation among components, target points and pathways. METHODS The traditional Chinese medicine pharmacology component analysis system platform(BAT-MAN) database to obtain the target gene of Zengye decoction. Cytoscape 3.5.1 software was used to build the active component-target network, and the STRING platform was used to build the target protein interaction network. GO bioprocess enrichment and clustering to analysize of interacting target genes by BiNGO and MCODE plug-ins. With the help of the biological information database annotation(DAVID) analysis of KEGG signal pathway on target. RESULTS The 23 pharmacodynamics components in Zengye decoction acted on 268 potential targets, and the cluster analysis of 862 GO biological processes yielded 23 sub-clusters, such as substance synthesis metabolism, oxidative stress, cell ion balance, inflammatory reaction, and coagulation regulation. The enrichment of KEGG signal pathways mainly involved 22 main signal pathways, such as metabolism, neurotransmitters, amino acid synthesis, signal transduction, cardiovascular and others. CONCLUSION This study reveals the active ingredients, target and multi-dimensional pharmacological mechanism of Zengye decoction, and provides new clues for the development of its clinical therapeutic value.