Abstract: OBJECTIVE To study the chemical ingredient from the root and stem of Zanthoxylum dissitum Hemsl.. METHODS The chemical ingredient were isolated and purified by silica gel column chromatography and preparative liquid chromatography, and the structures were elucidated based on the spectral data. RESULTS Ten compounds were obtained from the fraction of ethanol extract and identified as protopine (1), allcryptopine (2), canadine (3), chelerythrine (4), dihydrosanguinarine (5), dihydrochelerythrine (6), 6-acetonyldihydrosanguinarine (7), parahydroxyben-zaldehyde (8), isovanillin (9), syringaldehyde (10). CONCLUSION Ten compounds are isolated from this plant for the first time.