Abstract: Acacetin is a natural flavonoid which is widely found in nature. It can be extracted from wattle, acacia tree, thistle, airplane grass, Buddleja officinalis, chrysanthemum, and it is used in medical research and cosmetics industry. Acacetin has been found to have multiple biological activity, such as antioxidation, anti-inflammatory, antibacterial, anti osteoporosis, anti-tumor, immune regulation, and protect the heart and nervous system which may be of great help to the health of human beings. This paper reviews the relevant literatures about acacetin in China and abroad, summarizes its pharmacological functions and applications, and provides a reference for further research and development of acacetin.