Abstract: OBJECTIVE To study the effect of pine leaves extract on uric acid production and excretion, liver function, blood lipid level in hyperuricaemia rats. METHODS The 60 SD male rats were randomly divided into normal group, model group, allopurinol group, pine leaves extract groups (10.0, 5.0, 2.5 g·kg^-1). The rats in normal group were given with water, and the rats in other groups were given with lipid emulsion to establish hyperuricemia model in the morning and given with allopurinol and pine leaves extract in the afternoon for 9 weeks, except for normal and model groups. The general behavior, food intake and body weight of rats were observed, the serum indexes such as SUA, SCR, BUN, ALT, AST, TC, LDL-c and the urine indexes such as UUA, UCR, UV, 24 h UUA, FEUA, CUr, CCr were determined, and XOD, ADA, HGPRT enzyme activity of serum and liver were tested after the last administration. RESULTS The lipid emulsion could increase the serum indexes such as SUA, SCR, BUN, ALT, AST, TC, LDL-c and XOD, ADA enzyme activity of serum and liver, meanwhile decrease the urine indexes such as UUA, UCR, UV, 24 h UUA, FEUA, CUr, CCr and liver HGPRT enzyme activity of hyperuricemia rats, and the rats lost weight obviously. However, pine leaves extract could reduce the serum indexes such as SUA, SCR, BUN, ALT, AST, TC, LDL-c, and XOD, ADA enzyme activity of serum and liver, meanwhile increase the urine indexes such as UUA, UCR, UV, 24 h UUA, FEUA, CUr, CCr and liver HGPRT enzyme activity. CONCLUSION Pine leaves extract has obvious anti-hyperuricemia effect by reducing uric acid production and increasing uric acid excretion in hyperuricemia rats, and it has the function of protecting liver and regulating blood lipid.