阿里红中齿孔酸和总三萜酸的含量测定

Determination of Eburicoic Acid and Total Tritepenoidic from Fomes Officinalis Ames

摘要: 目的建立阿里红中齿孔酸及其总三萜酸的含量测定方法。方法采用紫外-可见分光光度法测定总三萜酸含量；采用HPLC测定齿孔酸含量。色谱柱为RP-C₁₈柱（4.6 mm×250 mm，5 μm）；流动相为乙腈-0.4%磷酸（68∶ 32），检测波长210 nm，流速1.0 mL·min^-1，柱温30℃。结果 3批阿里红药材中齿孔酸含量分别为2.3%，2.6%，3.1%；总三萜酸含量分别为27.2%，32.1%，35.9%。结论所建立的分析方法准确、简便，可为定量评价阿里红药材的质量提供依据。

Abstract: OBJECTIVE To determine the content of total tritepenoidic and eburicoic acid from Fomes officinalis Ames. METHODS The content of total tritepenoidic was determined by ultraviolet spectrophotometry. The content of eburicoic acid was determined by HPLC. The analysis was performed on a RP-C₁₈ column (4.6 mm×250 mm, 5 μm) with acetonitrile-0.4% phosphoric acid (68:32) as mobile phase at a flow rate of 1.0 mL·min^-1, and temperature was 30℃ The detection of wavelength was set at 210 nm. RESULTS The contents of eburicoic acid in 3 batches of Fomes officinalis Ames were 2.6%, 2.3%, 3.1%, and the contents of total tritepenoidic were 32.1%, 27.2%, 35.9%, respectively. CONCLUSION The method is suitable for the determination of tritepenoidic components from Fomes officinalis Ames.