UHPLC同时测定进口西洋参4个化学成分的含量

Simultaneous Determination of Four Constituents in Imported Panax Quinquefolium L. by UHPLC

摘要: 目的建立UHPLC同时测定进口西洋参中4个活性成分（人参皂苷Rg₁、人参皂苷Re、人参皂苷Rb₁和人参皂苷Rd）含量的方法。方法采用Zorbax SB C₁₈（2.1 mm×100 mm，1.8 μm）色谱柱，以乙腈为流动相A，水为流动相B，梯度洗脱，流速0.5 mL·min^-1，柱温30℃，检测波长为203 nm。结果 4种成分的分离度良好，且在各自浓度范围内呈现良好的线性关系（r≥0.999 9），平均回收率为95.7%~100.3%（RSD1.4%~1.8%）。结论该方法操作简单，缩短了分析时间，重复性好，为评价进口西洋参的质量提供了可靠的方法。

Abstract: OBJECTIVE To establish a UHPLC method for simultaneous determination of four active components (ginsenoside Rg₁, ginsenoside Re, ginsenoside Rb₁ and ginsenoside Rd) in imported Panax quinquefolium L.. METHODS An Agilent Zorbax SB C₁₈ column (2.1 mm×100 mm, 1.8 μm) was used, with mobile phase of acetonitrile as mobile phase A and water as mobile phase B (gradient elution) at a flow rate of 0.5 mL·min^-1, column temperature was 30℃ and detector wavelength was 203 nm. RESULTS The four components showed good separation and linearity within the tested range. The mean recoveries were in the range of 95.7%-100.3% (RSD 1.4%-1.8%). CONCLUSION It provides a rapid and reliable UHPLC method which is suitable for the quality control of Panax quinquefolium L..