Abstract: Single-cell sequencing provides information, such as heterogeneity, that is omitted by bulk sample sequencing. However, since cell growth and development are regulated by many factors, relying on genotype or phenotype information alone is not able to comprehensively and accurately explain the problem. Co-detection of single-cell DNA-Seq and RNA-Seq offers a strong tool for researchers on the aspect of the relationship between gene variations and phenotype variations. Up to present, there are strategies described below have been employed:whole cell lysis (e.g.:G&T-Seq, DR-Seq), plasma membrane lysis (e.g.:SIDR, microfluidics platform method) and others (e.g.:Simul-Seq, Gel-Seq). This review will introduce and compare these methods in addition to the prediction of their research trends.