Abstract: OBJECTIVE To establish quality standards of Ginseng Leptoradix. METHODS Empircal and microscopic identification methods were adopted to observe morphological and histological characters. The contents of water and total ash were analyzed according to the methods of Chinese Pharmacopoeia (2015). Ginsenoside constituent (Rb₁, Re, Rf, Rg₁) and reference herbs were used to identify materia medica of Ginseng Leptoradix. The ginsenoside constituent were analyzed by HPLC, Ginsenoside constituent (Re, Rg₁, Rb₁, Rb₂) was used as reference substances. The determination method was performed on a Sepax BR-C₁₈ column (4.6 mm×250 mm, 5 μm) with acetonitrile-water as the mobile phase, which was a multi-step acetonitrile-water gradient run. The detection wavelength was 203 nm. RESULTS The macroscopical identification, microscopic features and TLC methods were proper. The average congtents of water, total ash and ginsenoside constituent (Re and Rg₁, Rb₁, Rb₂) were 11.0%, 4.6%, 0.86%, 0.54% and 0.31%, respectively. CONCLUSION The quality standards established based on the research results, traditional experience and the production status of Ginseng Leptoradix in Jilin Province, which is suitable for the quality evaluation of Ginseng Leptoradix.