Abstract: OBJECTIVE To establish quantitative determination method of the protocatechuic acid, chlorogenic acid, vanillic acid, naringin, rutin, quercetin, luteolin, kaempferol and apigenin in Phragmites communis (Cav.) Trin. ex Steud from Yuanjiang by HPLC. METHODS The chromatographic separation was performed on a Diamonsil C₁₈ column (250 mm×4.6 mm, 5 μm) kept at 35℃, using methanol-0.1% formic acid aqueous as the mobile phase at a flow rate of 1 mL·min^-1 through gradient elution, and the detection wavelength was set at dual wavelength (phenolic acids:283, 327 nm; flavonoids:283, 335 nm), the injection volume was 10 μL. RESULTS The three phenolic acids and six flavonoids were isolated at 25 min and 50 min, the method exhibited linear range of 14.8-500 μg·mL^-1 (r=0.999 0-0.999 7), the limits of detection (LOD) was 0.006 3- 0.053 9 μg·mL^-1, the limits of quantification (LOQ) was 0.021 1-0.179 7 μg·mL^-1 and the recovery rate was 98.73%-102.44%, RSD was 1.27%-2.20% (n=6). CONCLUSION The method is accurate, sensitive, reproducible, and can be used to detect and quantitative the nine components in Phragmites communis (Cav.) Trin. ex Steud.