Abstract: OBJECTIVE To establish HPLC fingerprint method of Citrus changshan-huyou, and evaluate its quality combined with cluster analysis. METHODS The HPLC method was adopted with Extend XDB C₁₈(250 mm×4.6 mm, 5 μm) column, isocratic elution with the mobile phase was acetonitrile-0.1%formic acid solution, column temperature was 30℃, the wavelength was 330 nm, the flow rate was 1.0 mL·min^-1. RESULTS The reference HPLC fingerprints were set up through 11 batches of C.changshan-huyou, 19 common peaks were defined in fingerprints. Using MS/MS information, together with the reference standards and literatures, 9 common peaks were identified. Similarity analysis and hierarchical cluster analysis were applied to study the HPLC characteristic fingerprint and chemical pattern recognition. There were high similarity in different samples, similarity were 0.995-1.000, and were clustered together, the quality of C.changshan-huyou were consistent, others with high disparities, were clustered together. CONCLUSION This method is simple, rapid and high reproduction quality. It can provide the foundation for quality control of C.changshan-huyou.