Abstract: OBJECTIVE To study the protective effects and anti-inflammation mechanism of grape seed procyanidin(GSP) on alcoholic liver injury in mice. METHODS Sixty male ICR mice were randomly divided into six group:normal group. model group (56% alcohol), silymarin group(56% alcohol +90 mg·kg^-1silymarin), high, medium, low GSP dose group(56% alcohol+400, 200, 100 mg·kg^-1GSP). Eight weeks later, serum alanine aminotransferase(ALT) and aspartate aminotransferase (AST) level, malondialdehyde (MDA) contents, the superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity in liver were measured by spectrophotometry. The contents of NF-κB, TNF-α, and IL-1β in liver were analyzed by ELISA. HE observe the pathological change in the hepatic tissue. RESULTS Compared with the model groups, GSP in the treatment group decreased the levels of serum ALT and AST and the contents of MDA, NF-κB, TNF-α, IL-1β (P<0.05), but increased the activity of SOD and GSH-Px in the hepatic tissue. CONCLUSION GSP can reduce the damage of liver tissue in mice with alcoholic liver injury, by reducing oxidative stress and inflammatory reaction.