Abstract: OBJECTIVE To establish the internal control standard of Fufang Fuqing lotion, which makes the clinical medicine more reasonable and safe. METHODS dandelion and paeoniae rubra were identificated by TLC. Simultaneous determination of paeoniflorin, isochlorogeni acid A and caffeic acid in Fufang Fuqing lotion by HPLC. The samples of Fufang Fuqing lotion were separated on Inertsil ODS-SP column(4.6 mm×250 mm, 5 μm). The mobile phase was set as acetonitrile-0.02% phosphoric acid(11:89), and the flow rate was 1.0 mL·min^-1. The detection wavelength were set at 230 and 323 nm. RESULTS In TLC could be detected paeoniae rubra radix and dandelion characteristics of the spots. The calibration curves of peaoniflorin, isochlorogenic acid A and caffeic acid showed a good linear relationship over the range of 14.06-225 μg·ml^-1(r=0.999 9), 7.5-120 μg·ml^-1(r=0.999 8) and 2.5-40 μg·ml^-1(r=0.999 7). The average recovery rate were 94.5%, 98.6%, 99.2% with RSD 0.98%, 1.66%, 0.65%. CONCLUSION The established TLC identification method is simple, accurate, and the content of HPLC method is specific, which can be used for quality control of Fufang Fuqing lotion.