Abstract: OBJECTIVE To establish the HPLC fingerprint and study on rapid extraction and content determination of curculigoside in Curculiginis Rhizoma, provide evidence for quality control. METHODS The orthogonal design was used for ASE 350 condition optimization, and the content of curculigoside was determined by HPLC, using Thermo Syncronis C₁₈(100 mm×3 mm, 3 μm) column, acetonitrile-0.1% phosphoric acid as mobile phase by gradient elution, the flow rate was 0.5 mL·min^-1, the column temperature was 40℃, detection wavelength was 285 nm. The data analysis was performed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine (Version 2.0). RESULTS The optimal extraction conditions were extracting two times at 100℃ and static extraction time was 5 min. There were fifteen common peaks in the fingerprint. The similarity of fingerprints of 20 batches of Curculiginis Rhizoma were all >0.9. CONCLUSION The method is rapid, accurate, and can be used to control quality evaluation for Curculigins Rhizoma.