Abstract: OBJECTIVE To study the regulation effects of soil treatments on continuous cropping obstacle in Atractylodes macrocephala.METHODS The effects of soil treatment were studied with calcium cyanamide, lime, biofertilizer and silkworm fementation, by investigating the seedling survival rate, plant height, crown width and output, and determining the content of atractylenolide Ⅰ and Ⅲ by HPLC.RESULTS Lime nitrogen, Silkworm excrement ferment fertilizer and biological fertilizer could improve the survival rate of continuous cropping of Atractylodes macrocephala by 106.98% to 219.49% in varying degrees, also could enhance the plant height of Atractylodes macrocephala with different degree by 33.48%-52.01%. And the plant height in lime nitrogen group were always higher than that in no-lime-nitrogen group. The crown width was similar to plant height. The yield of Atractylodes macrocephala was increased by 180.70%-429.82%, and the yield was higher than that of lime nitrogen without application of lime nitrogen. The possible reason was that the nitrogen content in the soil was too high after the application of calcium cyanamide, resulting the plant height vigorous growth.CONCLUSION Before continuous cropping, using slaked lime to neutralizing sold acidity, then fertilizing silkworm fementation or biofertilizer can increase seedling survival rate, reduce the incidence rate and improve the medicinal material yield and quality effectively, so as to alleviate the continuous cropping obstacle of Atractylodes macrocephala to a certain extent.