Abstract: OBJECTIVE To investigate and compare the effect of Xiaoer Jianpi plaster recipe and its ingredients on the gastric and ileum smooth muscle of rats in vitro, and identify the herbs that promote gastrointestinal motility in this prescription, so as to lay a foundation for the study of its effective substances.METHODS The biological signal acquisition system was used to record the changes of the tension of the normal gastric and ileum smooth muscle of the rats, and the effects were compared.RESULTS The promoting effects of the whole recipe and its ingredients on the gastric muscle contraction:the whole recipe > clove > hawthorn > cinnamon, and the effect white pepper was not obvious. The promoting effects on the contraction of ileum smooth muscle:clove > the whole recipe > hawthorn, the effects of cinnamon and white pepper were not obvious. Evodia had a bidirectional regulation of the gastric and ileum smooth muscle contraction, it could promote in the small dose, while the dose increased, it had an inhibitory effect.CONCLUSION The Xiaoer Jianpi plaster recipe can promote gastric and ileum smooth muscle contraction; in the single ingredient, there are notable effects of clove and hawthorn, evodia has a bidirectional regulation; clove and hawthorn are the main ingredients that promote the gastrointestinal motility in this prescription.