Abstract: OBJECTIVE To examine the differentially expressed genes and pathways, and detected the different gene expression profiles by analyze the microarray data of both HPV-positive and HPV-negative oropharyngeal cancer patients.METHODS First selected the gene expression profiles of HPV-positive and HPV-negative oropharyngeal cancer patients in GEO database. Then, screened the differentially expressed gene cluster and pathways of HPV-positive and HPV-negative oropharyngeal cancer by GO gene functional annotation and KEGG pathway enrichment analysis, and finally conducted the visualization analysis of protein interaction network.RESULTS HPV infection had affected the biological characteristics of oropharyngeal cancer, and significantly altered some important tumor signaling pathways, such as Wnt and PI3k-Akt signaling pathways.CONCLUSION This study has employed bioinformatics method from various perspectives to define the gene expression characteristics of different HPV infection in oropharyngeal cancer, and provides theoretical basis for accurate treatment of oropharyngeal cancer.