Abstract: OBJECTIVE To study anti-inflammatory effect of the metabolites of endophytes isolated from Glycyrrhiza uralensis in vivo and in vitro.METHODS RAW264.7 cells were inoculated onto a 96-well plate. The model group was added with 1 mg·L^-1 LPS for culture, and each test drug group was added with Glycyrrhiza water extracts or the fermentation products of endophytes (10, 20, 40 mg·L^-1) for 2 h of treatment, then LPS (1 mg·L^-1) was added for stimulation. The content of nitric oxide (NO), Interleukin-1β (IL-1β) and Prostaglandin E₂ (PGE₂) were measured. Ninety Wistar rats were randomly divided into 9 groups, and each group was given the corresponding drug by gavage administration once per day for 5 d. Except the blank group, the other groups were intravenously injected with LPS (1 mg·kg^-1) to establish an acute pneumonia model, while the blank group was injected with the same volume of normal saline. After 6 h, blood was collected from rat femoral artery to detect peripheral white blood cell counts and the contents of serum TNF-α, IL-6 and IL-10.RESULTS Compared with LPS model group, when the mass concentration was maintained at 40 mg·L^-1, the contents of NO, PGE₂ and IL-1β were significantly reduced in extractum Glycyrrhiza group and endophytes fermentation products groups(JTZB006, JTZB018, JTZB059, JTZB060, JTZB063)(P<0.05 or P<0.01). The contents of PGE₂ in JTZB006, JTZB060 were significantly lower than those of extractum Glycyrrhiza group at 40 mg·L^-1. Compared with model group, the white blood cell counts and the contents of TNF-α and IL-6 were significantly reduced in positive control group, Glycyrrhiza water decoction group, 3 groups of the fermentation products of endophytes. The contents of IL-10 in JTZB006, JTZB060 and JTZB063 significantly higher than those of Glycyrrhiza water decoction group in rat serum (P<0.05 or P<0.01).CONCLUSION JTZB006, JTZB060 and JTZB063 have anti-inflammatory effects in LPS induced inflammatory model.