小春花药材质量标准研究

辛文秀; 钟里科; 张轶雯; 方罗; 郑小卫; 丁海樱; 黄萍

doi:10.13748/j.cnki.issn1007-7693.2018.04.018

小春花药材质量标准研究

Study on Quality Standards for Sceptridium Ternatum

摘要

摘要: 目的制定并考察小春花药材质量标准，为进一步开发小春花药材提供基础。方法采用紫外-可见分光光度法建立小春花中总黄酮的含量测定方法；采用HPLC同时测定小春花药材中槲皮素和山奈酚的含量。结果建立小春花总黄酮的测定方法，槲皮素为在30.23~181.40 μg·mL^-1呈良好线性关系，R²=0.998 4；确定药材中槲皮素、山奈酚的含量测定方法，槲皮素在2.64~21.12μg·mL^-1呈良好的线性关系，R²=0.999 8，山奈酚在2.00~16.00μg·mL^-1呈良好的线性关系，R²=0.999 9；小春花总黄酮以槲皮素计不得<0.2‰，山奈酚计不得<0.6‰。结论本研究对小春花药材进行质量控制，提升并建立了新的小春花药材质量标准。

Abstract: OBJECTIVE To establish the quality standards for Sceptridium ternatum, and lay the foundation for its further development. METHODS The content of total flavonoids was assayed by UV-Vis spectrophotometry. The contents of quercetin and kaempferol were determined by HPLC. RESULTS There was a good linear relationship between the absorbance values and the quercetin concentration when it was in the range of 30.23-181.40 μg·mL^-1(R²=0.998 4). The quercetin and kaempferol were in good linear relationships in the range of 2.64-21.12 μg·mL^-1(R²=0.999 8) and 2.00-16.00 μg·mL^-1(R²=0.999 9), respectively. The quercetin/kaempferol content in Sceptridium ternatum should not be less than 0.2‰ and 0.6‰, respectively. CONCLUSION The quality control standard for Sceptridium ternatum is modified and a new one is provided to guarantee the quality of raw medical materials.

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