Abstract: OBJECTIVE To discuss the pharmaceutical care points in intracranial infection of multidrug-resistant organism and share the experience in anti-bacterial drug usage by the participation in therapy of a patient with intracranial infection after the operation of traffic tumor. METHODS Clinical pharmacist monitored the whole process of anti-infective treatment. Providing suggestion to the administration of drugs, analyzing the condition of the patient and factors related to infection. Giving the proposal of amikacin administrated via intraventricular and intrathecal injection according to various literature. RESULTS The proposal was adopted by the doctor and the intracranial infection was controlled. The patient was discharged from hospital without any injury of organ function and adverse reaction. CONCLUSION Removing the infected factors and adjusting anti-bacerial drugs according to drug sensitivity tests in time is important. Local use of antibiotics can be enabled and disabled under the care of clinical pharmacists.