杨梅醇与5-对氟苄氧基杨梅醇的制备及其对人肝癌HepG<sub>2</sub>细胞活性的影响

杨梅醇与5-对氟苄氧基杨梅醇的制备及其对人肝癌HepG₂细胞活性的影响

摘要: 目的体外观察杨梅醇与5-对氟苄氧基杨梅醇（myricanol 5-fluorobenzyloxy ether，5FEM）对人肝癌细胞HepG₂活性的影响。方法制备杨梅醇与5FEM，杨梅醇（25，12.5，6.25 μg·mL^-1）、5FEM（25，12.5，6.25 μg·mL^-1）作用于HepG₂细胞，实时细胞分析系统记录96 h细胞指数，观察杨梅醇与5FEM对HepG₂细胞增殖和迁移的影响。结果杨梅醇与5FEM对HepG₂细胞的增殖和迁移均有较好的抑制作用，且呈现较好的量效关系，相等剂量下，5FEM的作用优于杨梅醇。结论杨梅醇与5FEM能有效抑制HepG₂细胞的增殖和迁移。

Abstract: OBJECTIVE To observe the activity of myricanol and myricanol 5-fluorobenzyloxy ether (5FEM) on liver cancer of cell HepG₂in vitro. METHODS Myricanol (25, 12.5 and 6.25 μg·mL^-1) and 5FEM (25, 12.5 and 6.25 μg·mL^-1) were prepared to act on HepG₂ cells, real time cellular analysis was used to record 96 h cell index (CI) to observe the effect of Myricanol and 5FEM on the proliferation and migration of HepG₂ cells. RESULTS Myricanol and 5FEM could inhibit the proliferation and migration of HepG₂ cells, showing a dose-effect curve. The effect of 5FEM was better than myricanol at the same dose. CONCLUSION Myricanol and 5FEM can effectively inhibit the proliferation and migration of HepG₂ cells.