Abstract: OBJECTIVE To carry out the pharmacological efficacy in vivo and mechanism research based on clear component of alkaloids of Sophorae Tonkinensis Radix et Rhizoma. METHODS Hepatocarcinoma model rats were induced by diethylnitrosamine (DEN), SD rats were randomly divided into six groups:normal control group, hepatocarcinoma model group, cyclophosphamide group (0.01 g·kg^-1)and low, medium, high dose group of alkaloids (0.036, 0.108, 0.324 g·kg^-1), and seven weeks later treated with corresponding drugs for nine weeks. Observe physiological indexes and pathologic section of each group, the levels of AFP in serum were measured and the expression levels of VEGF、PI3K、PTEN mRNA were analyzed by RT-PCR. RESULTS Compared with model group, the alkaloids of Sophorae Tonkinensis Radix et rhizoma could significantly reduce the content of AFP in serum(P<0.05), Histological examination demonstrated that the number of liver cancer nodes in alkaloids groups were lower than that of model group, at the same time, the alkaloids of Sophorae Tonkinensis Radix et rhizoma could down-regulate the expression of VEGF, PI3K mRNA and up-regulate the expression of PTEN mRNA in liver tissue. CONCLUSION The clear pharmacological effects of liver cancer-suppression in vivo is produced by the alkaloids of Sophorae Tonkinensis Radix et rhizoma. Improved immunity and extended lifetime; Its mechanism is similar with cyclophosphamide, regulation VEGF, PI3K, PTEN expression. Provide a reference basis for alkaloids of Sophorae Tonkinensis Radix et rhizoma for the clinical application.