Abstract: OBJECTIVE To investigate the effect of flight stress on the physiological and biochemical parameters of rats in the processes of acute expose to high altitude. METHODS To compare the difference of physiological and biochemical parameters between flying from plain area (Shanghai) to plain area (Xi'an) and to high plateau (Ma Do). RESULTS Compared with Shanghai group, the aspartate aminotransferase, alanine aminortransferase, alpaline phosphatase, urea nitrogen, uric acid, glucose, total protein of acute exopose to MaDo group were significantly increased 130%, 117%, 17.91%, 52.27%, 331%, 97.61% and 29.75%(P<0.01). While lactate dehydrogenase, creatinine were significantly reduced 68.16% and 48.21% after acute exposed from plain area to high altitude(P<0.01). The hematocritg, concentration of total hemoglobin, serum sodium, serum chlorine were significantly increased 9.30%, 8.57%, 2.36% and 10.24% while buffer base, buffuer excess, carbon dioxide tension of arterial blood, oxygen saturation of arterial blood, oxygen tension of arterial blood were significantly reduced 207%, 380%, 29.76%, 7.42%, 43.29% after acute exposed from plain area to high altitude(P<0.05 or P<0.01). The indicators after acute exposed from plain area to plain area were insignificantly on statistics. CONCLUSION Flight stress has no effect on the physiological and biochemical parameters of rats in the progress of acute expose to high altitude, it can molding an experimental model by acute flying from plain area to high altitude to investigate the hypoxia effects on pharmacokinetics.