Abstract: OBJECTIVE To observe the protective effects of quercetin on the BV-2 microglia injury induced by LPS and study the possible mechanism. METHODS BV-2 cells were cultured in vitro and LPS was used to stimulate the inflammatory model of BV-2 cells. The cells were divided into five groups:blank control group, model group, quercetin treatment groups (10, 20, 40 μg·mL^-1). CCK-8 kit was used to determine the relative survival rate of BV-2 cells and ELISA kits were used to measure the expression levels of IL-6 and TNF-α. Western blot was used to analysis the expression levels of iNOS, COX-2, Traf6, p65 and p-p65. RESULTS The BV-2 cells injury model was induced by LPS. The BV-2 cells relative survival rates after treatment for different concentrations of quercetin were significantly increased compared to model group, while the expression levels of inflammatory cytokines IL-6 and TNF-α were significnatly decreased compared with model group. The results of Western blot showed that the expression levels of iNOS, COX-2, Traf6 and p-p65 were significantly decreased after treatment with quercetin. CONCLUSION Quercetin can significantly inhibit the inflammatory response of BV-2 microglial cells induced by LPS via suppressing the activation of Traf6/p65 signaling pathway.