Abstract: OBJECTIVE To study the effect of polydatin(PD) on the expression of β-Catenin and GSK-3β protein in ovariectomy osteoporosis rats. METHODS The 60 female SPF rats were randomly divided into sham operation group, model group, PD low(5 mg·kg^-1·d^-1), middle(15 mg·kg^-1·d^-1) and high dose(45 mg·kg^-1·d^-1) groups (12 rats in each group, ig), every group were established by ovariectomy except sham group. After 12 weeks, the bone mineral density (BMD) of these rats was detected by dual energy X-ray absorptionmetry, ELISA method was used to detect serum estradiol(E2), bone alkaline phosphatase(ALP), tartrate resistant acid phosphatase(TRAP) and other biochemical indicators of the level; western blotting was used to detect the expression of β-Catenin and GSK-3β protein; qRTPCR was used to detect the expression of β-Catenin and GSK-3β mRNA. RESULTS Compared with model group, PD groups increased BMD, E2 significantly(P>0.01), decreased ALP, TRAP level(P>0.01) and increased the expression of β-Catenin protein and mRNA(P>0.01); decreased the expression of GSK-3β protein and mRNA (P>0.01). CONCLUSION PD has therapy effects in ovariectomy osteoporosis rats. Its mechanism could be relative with increasing the expression of β-Catenin protein and decreasing expression of GSK-3β.