百贝益肺胶囊含量测定的方法改进

孙荣飞; 张金玺; 赵晓星; 者从章

doi:10.13748/j.cnki.issn1007-7693.2017.12.021

百贝益肺胶囊含量测定的方法改进

Improvement of Content Determination of Baibei Yifei Capsule

摘要

摘要: 目的增加评价指标，建立科学、合理的百贝益肺胶囊含量测定方法。方法 C₁₈小柱纯化样品，优化色谱条件为：Vp-ODS色谱柱（150 mm×4.6 mm，5 μm）；柱温：20℃；变动流速；流动相：乙腈（A）-水（B），梯度洗脱；进样量：10 μL；检测波长：203 nm。结果所测成分三七皂苷R₁、人参皂苷Rg₁、人参皂苷Rb₁分别在40.24~241.44（r=0.999 6），91.50~549.00（r=0.999 9），35.72~241.32（r=0.999 8）μg·mL^-1内线性关系良好；加样回收率为94.15%~99.81%，RSD为1.12%~1.94%。结论该方法准确，重复性良好，可为修订标准提供依据。

Abstract: OBJECTIVE To augment evaluation index, and establish a scientific and reasonable method for the content determination of Baibei Yifei capsule. METHODS Samples were purified by C₁₈ column, optimized chromatographic conditions:Vp-ODS column (150 mm×4.6 mm, 5 μm); column temperature:20℃; fluctuan flow rate; the mobile phase acetonitrile (A)-water (B), gradient elution. The injection volume was 10 μL. The detection wavelength was 203 nm. RESULTS The contents of notoginsenoside R₁, ginsenoside Rg₁, ginsenoside Rb₁ were linear in 40.24-241.44(r=0.999 6), 91.50-549.00 (r=0.999 9), 35.72-241.32(r=0.999 8)mg·mL^-1, respectively. The recovery rate was 94.15%-99.81%, RSD was 1.12%-1.94%. CONCLUSION The method is accurate and reproducible, which can provide the basis for the revision of the standard.

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