响应面法优化胡柚幼果中柚皮苷与新橙皮苷的提取工艺

Response Surface Methodology for Optimization of Extraction Process of Naringin and Neohesperidin from Huyou fruitlet

摘要: 目的优选胡柚幼果中柚皮苷与新橙皮苷的提取工艺。方法在单因素试验的基础上，对乙醇浓度、料液比、提取时间3个因素进行响应面Box-Behnken中心组合设计优化，得到最佳提取工艺。结果最佳提取工艺条件为乙醇浓度60%、料液比1：10、提取时间1.8 h、回流提取2次，在此条件下柚皮苷与新橙皮苷的总得率为23.44%。结论本提取工艺方法简单、合理、稳定，为工业化生产提供了一定的依据。

Abstract: OBJECTIVE To optimize the extraction process of naringin and neohesperidin from huyou fruitlet. METHODS Based on the single factor experiment, Box-Benhnken three factors (ethanol concentration, solid-liquid ratio and extraction time) experiment by response surface design was carried out. RESULTS The optimum conditions was as follows:solid to liquid ratio 1:10, 60% ethanol, extraction time 1.8 h and extraction two times. Under these conditions, the total yield of naringin and neohesperidin from huyou fruitlet was 23.44%. CONCLUSION The method is simple, reasonable, feasible and provide a experiment basis for its industrial production.