Abstract: OBJECTIVE To study the protective effect of polysaccharides from different concentrations of ethanol on acute liver injury in mice. METHODS Polysaccharide was obtained by ethanol precipitation at different concentrations, Establishment of acute liver injury mice induced by D-Galactosamine, Elisa method for detection of alanine aminotransferase (ALT), aspartate aminotransferase(AST), tumor necrosis factor alpha (TNF-alpha) and interleukin-6(IL-6), interleukin-10(IL-10) expression in serum and SOD, CAT, GSH-Px, GSH expression in liver. Liver HE staining was used to examine histopathology. The expression of Bcl-2, Bax and Caspase-3 mRNA in liver tissue was detected by qPCR In order to determine the effective part of polysaccharide on acute liver injury in mice. RESULTS Each concentration ethanol precipitate of the polysaccharide precipitated of antrodia camphorata polysaccharide had hepatoprotective effect. 90% alcohol significantly reduced ALT, AST in mice and the effect was better than the other groups (P<0.05). Four kinds of polysaccharides could significantly reduce the expression of tumor necrosis factor (TNF-α), interleukin-6 (IL-6), in serum and Bax and Caspase-3mRNA in liver tissue, improve the interleukin -10 (IL-10) expression in serum and SOD, CAT, GSH-Px, GSH protein expression、Bcl-2mRNA in liver tissue (P<0.05). The effect of PW90 polysaccharide was better. CONCLUSION Different concentrations of ethanol precipitation of the polysaccharide has a protective effect on acute liver injury, 90% ethanol precipitated polysaccharide was better than other ethanol concentration.