知柏地黄丸的UPLC指纹图谱研究

Study on UPLC Fingerprint of Zhibai Dihuang Pills

摘要: 目的建立知柏地黄丸UPLC指纹图谱的研究方法。方法采用Waters Acquity BEH C₁₈色谱柱（2.1 mm×100 mm，1.7 μm），流动相为乙腈-0.1%磷酸，梯度洗脱，检测波长236 nm，流速0.4 mL·min^-1，柱温40℃，以丹皮酚作为参照物。结果在12 min内完成指纹图谱分析，标出12个共有峰，对6个色谱峰进行了归属，并进行了相似度评价。结论 UPLC简便可行，建立的指纹图谱可用于知柏地黄丸的质量控制。

Abstract: OBJECTIVE To develop a method for fingerprint study of Zhibai Dihuang pills by UPLC. METHODS The chromatographic condition was as follows:Waters Acquity BEH C₁₈ column(2.1 mm×100 mm, 1.7 μm), using acetonitrile-0.1% H₃PO₄ as mobile phase, with a flow rate of 0.4 mL·min^-1, the detective wavelength was set at 236 nm and the column temperature was 40℃. Paeonol was used as the reference substance. RESULTS The analysis time was 12 min. There were 12 common peaks in the fingerprints, 6 peaks were identified and the similarity of the fingerprint was compared. CONCLUSION The method is simple, feasible, and can be used for qulity control of Zhibai Dihuang pills.