Abstract: OBJECTIVE To explore the effect and mechanism of total flavonoids extracts of Galium verum L.(TFG) on CCl₄-induced liver injury in mice. METHODS Sixty KM mice were randomly divided into the normal group, the model group, the positive control group and TFG group. The mice in each treatment group were given corresponding drugs for 6 d. Except the normal group, the other groups were injected intraperitoneally with 6% CCl₄ for 24 h, HE staining was used. Activitise of serum aspartate aminotransferase(AST), alanine aminotransferase(ALT) and levels of albumin superoxide dismutase(SOD), glutathione(GSH-Px), and malondialdehyde (MDA) in liver and kidney tissue were detected, levels of serum TNF-α and IL-6 were analyzed. Fifty KM mice were randomly divided into normal group, model group, TFG group(200 mg·kg^-1), anti-TNF-α monoclonal antibody(40 mg·kg^-1)+TFG(200 mg·kg^-1) group, anti-IL-6 monoclonal antibody(5 mg·kg^-1)+TFG(200 mg·kg^-1) group. The mice in each treatment group were given corresponding drugs for 6 d. Except the normal group, the other groups were injected intraperitoneally with 6% CCl₄ for 24 h. Finally, activitise of serum AST and ALT were measured. RESULTS Compared with the model group, TFG could improve CCl₄-induced liver injury in mice, significantly decrease activitise of serum AST, ALT and level of MDA in liver and kidney, increase activities of SOD and content of GSH in liver and kidney, significantly decreased the levels of serum TNF-α and IL-6(P<0.05 or P<0.01). Anti-IL-6 and TNF-α monoclonal antibody could significantly decrease activities of serum AST and ALT(P<0.05). CONCLUSION TFG can effectively improve the CCl₄-induced liver injury in mice, which may be related to its ability to improve antioxidant, inhibit lipid peroxidation, protect the integrity of the cell membrane and mitochondrial membrane, while reducing the release of IL-6 and TNF-α.