Abstract: OBJECTIVE To prepare thermosensitive in-situ gel of norcantharidin (NCTD) and to explore its inhibitory effects on hepatoma. METHODS NCTD thermosensitive in-situ gel was prepared using poloxamer 407 and poloxamer 188. Tumor growth inhibition caused by NCTD thermosensitive in-situ gel was examined on tumor-bearing mice using mouse H22 hepatoma cells to build a solid tumor model. RESULTS The prepared in-situ gel displayed a white transparent liquid state at room temperature but a white transparent gel state at gelling point, which was 34℃. Significant slow release characteristic was presented at gel state; anti-tumor activity experiment in vivo suggested significant inhibition effects on tumor growth in solid hepatoma bearing mice. CONCLUSION NCTD thermosensitive in-situ gel owns stable properties and good antitumor activities.