Abstract: OBJECTIVE To establish a determination method of Pb, Cd, Cu, As and Hg in breeding state of Eisenia foetida Savigny, and the contents of Pb, Cd, Cu, As and Hg were quantitatively determined in 6 batches of Eisenia foetida savigny.. METHODS The sample was digested by microwave, the content of Pb and Cd was measured by graphite furnace atomic absorption spectrometry (GFAAS), the content of Cu was measured by flame atomic absorption spectrometry (FAAS); the content of As and Hg was measured by atomic fluorescence spectrometry. RESULTS The recovery of Pb was 103.6%(RSD=6.4%), the recovery of Cd was 92.0%(RSD=7.7%), the recovery of Cu was 101.6%(RSD=8.1%), the recovery of As was 94.4%(RSD=4.7%) and the recovery of Hg was 93.6%(RSD=5.4%). CONCLUSION These methods are simple and rapid for operating. Moreover, the accuracy of these methods is high. Experiments show the method is suitable for the determination of the contents of Pb, Cd, Cu, As and Hg in Eisenia foetida Savigny.