Abstract: OBJECTIVE:To establish a rat model of hyperuricemia combined with nonalcoholic fatty liver disease by feeding modified high yeast high fat diet with oxonic acid potassium intragastric administration or subcutaneous injection. METHODS Forty-eight male SD rats were randomly divided into 4 groups:normal control group(Group A), high-fat control group (Group B), high yeast high fat diet with oxonic acid potassium salt intragastric administration group(Group C), high yeast high fat diet with oxonic acid potassium salt subcutaneous injection group(Group D), 12 rats in each group. Body mass, liver wet weight, UA, CHO, TG, ALT, AST, BUN and CREA were respectively observed in each group. And the pathological changes were analyzed. RESULTS After molding, the liver wet weight of the rats in Group C and Group D was significantly higher than that of Group A. The liver wet weight of Group C and Group B had no statistical difference (P>0.05). The liver wet weight of the rats in Group D was significantly lower than that of Group B (P<0.01). UA, CHO, TG, ALT, AST and CREA were significantly decreased after molding (P<0.05), but BUN was similar to Group A (P>0.05). Besides, after molding, range of fatty degeneration and inflammatory reaction in the liver tissue were increased compared with the Group A (P<0.01). While compared with Group A, there was no significant lesion in the renal tissue of rats after molding (P>0.05). CONCLUSION A rat model of hyperuricemia combined with nonalcoholic fatty liver disease was established successfully with no obvious kidney damage, which is similar to human pathogenesis and provide a more targeted animal model for the study of high uric acid combined with nonalcoholic fatty liver disease.