Abstract: OBJECTIVE To compare the expression level of drug-target proteins in patients' colorectal carcinoma tissue with Caco-2 cell line from human protein database to clarify the consistency. METHODS The protein expression levels of FDA-approved target-proteins involving in both colorectal carcinoma patients and cell lines were scored base on the intension and quantity of immunohistochemical stain from the database of Human Protein Atlas. And the protein expression between individual and cell line was compared. Then the consistency profiles of total proteins between individual and cell line were evaluated. Ultimately, proteins with well or poor consistent expression were identified analyzed by Gene ontology and KEGG enrichment. RESULTS The expression levels of 176 target proteins involving in both Caco-2 cell line and colorectal carcinoma patients(n=104) were obtained and analyzed. Almost 57.4% of proteins in individual patients were consistent to cell line, which was independently from individual characteristics, such as age, gender and tumor location. About 40% and 47% of total protein, included 47 and 36 proteins with entirely consistent and inconsistent profile, were well and poor consistent expression between patients and cell line, respectively. Those inconsistent proteins were enriched in the pathways related to various types of cancer, immune, extracellular matrix receptor interactions and cytoskeleton. CONCLUSION There is a significant difference in the target protein expression between Caco-2 cell line and colorectal tissue, the results suggest that the consistency is important to investigate cell as the model in drug preclinical development.